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Yes, catalase functions best at the same low ph and temperature as occurs in the mammalian tissue. No, catalase functions best at a much higher ph than in mammalian tissue and at a much lower temperature. Yes, catalase worked best at a neutral ph and temperature of 40 °c, both of which are close to mammalian tissue conditions. View bio enzymes lab. docx from biology 1321 at brooklyn college, cuny.